The endpoint titer was calculated as the reciprocal serum dilution giving O
The endpoint titer was calculated as the reciprocal serum dilution giving O.D 450 nm readings 2 and the background levels which was calculated using pre-bleed serum at the same dilutions. Western Blot Analysis For Western blot analysis, the S and RBD soluble proteins were separated in 12% sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel and transferred to… Read More »